RecombinantBiotinylatedHumanOX40/TNFRSF4/CD134Protein(PrimaryAmineLabeling),hFcTag性能參數(shù)分子別名(Synonyms)OX40Lreceptor;CD134;TNFRSF4;Ly-70表達區(qū)間及表達系統(tǒng)(Source)BiotinylatedHumanOX40/TNFRSF4/CD134Protein(PrimaryAmineLabeling)isexpressedfromHEK293withhFctagattheC-Terminus.ItcontainsLeu29-Ala216.[Accession|P43489]分子量大?。∕olecularWeight)TheproteinhasapredictedMWof46.8kDa.Duetoglycosylation,theproteinmigratesto72-75kDabasedonSDS-PAGEresult.(Endotoxin)Lessthan1EUperμgbytheLALmethod.純度(Purity)>95%asdeterminedbySDS-PAGE制劑(Formulation)Suppliedas0.22μmfilteredsolutioninPBS(pH7.4).儲存條件Theproductshouldbestoredat-85~-65℃for1yearfromdateofreceipt.Recommendtoaliquottheproteinintosmallerquantitieswhenfirstusedandavoidrepeatedfreeze-thawcycles.UbcH3還有兩個泛素結合位點UBS1(來自aa 205-215)和UBS2(來自aa 216-225),以及一個c端酸性尾部結構域,。Recombinant Mouse Fc gamma RIIB/CD32b Protein,His Tag
糖苷酶F(PNGaseF)是一種酰胺水解酶,,經過和平空間站伊麗莎菌克隆,,主要由腦膜炎膿桿菌等革蘭氏陰性菌分泌。N-糖苷酶F(PNGaseF)在酵母中重組表達(比活性:100000U/mL),,可以裂解由天冬酰胺連接的高甘露糖,、雜合和復雜的寡糖糖蛋白。PNGaseF的切割位點為糖蛋白內側N-乙酰葡萄糖胺(GlcNAc)和天冬酰氨殘基之間的酰胺鍵,,同時將酶解后蛋白上的天冬氨酰轉化為天冬氨酸,。本產品帶his標簽,常應用于抗體及其相關蛋白完全去糖基化,。另外,,我司還提供其他類型的糖苷酶,,包括酵母重組表達的N-糖苷酶F(比活性:750000U/mL),內切糖苷酶H,,內切糖苷酶S,。產品信息產品性質中文別名(Chinesesynonym)N-糖酰胺酶F;N-糖苷酶F英文別名(Englishsynonym)PNGaseF來源(Source)酵母重組表達分子量(Molecularweight)36kDa比活性(Specificactivity)100000U/mL緩沖液組分(Buffer)20mMTris-HClpH7.5,50mMNaCl,5mMEDTA,50%GlycerolRecombinant Canine S100B Protein,hFc Tag通過研究,,CDNF可防止6-羥基多巴胺(6-OHDA)誘導的多巴胺能神經元變性,,這可能對帕金森氏病有益。
產品簡介凝血酶是由大小分別為31KD和6KD的兩條肽鏈通過二硫鍵組成的一種絲氨酸蛋白水解酶,,可從動物血漿中利用已凝血酶原水解制得,,可催化纖維蛋白原(fibrinogen)水解釋放A肽和B肽,由此形成纖維蛋白單體,,單體進一步聚合,,在血小板、紅細胞和白細胞等參與下形成血凝塊,,常用于診斷學中凝血化驗,、凝血因子檢測、血液或血漿的脫纖維化等,;另外,,由于凝血酶切割序列專一,水解效率高,,因此在分子生物學,、生物化學或生物工程制藥研究中也常作為工具酶用于重組融合蛋白(包含凝血酶識別位點)的特異性斷裂。本品是從牛的血漿純化制得,,具有純度高,、比活高、不含有其他蛋白酶活性,、滅活病毒,、生產穩(wěn)定性好等特點。該酶適切割位點:A-B-Pro-Arg-▼-X-Y(其中,,A和B為疏水氨基酸,,X和Y為非酸性氨基酸),常見的識別序列為:1.Leu-Val-Pro-Arg-▼-Gly-Ser,。2.Gly-Arg-▼-Gly,。產品信息規(guī)格1KU/2KU
RecombinantBiotinylatedHumanENPP-3Protein,His-AviTag性能參數(shù)分子別名(Synonyms)NPP3;E-NPP3;PD-Ibeta表達區(qū)間及表達系統(tǒng)(Source)BiotinylatedHumanENPP-3ProteinisexpressedfromHEK293withHistagandAvitagattheN-Terminus.ItcontainsLeu48-Ile875.[Accession|O14638]分子量大小(MolecularWeight)TheproteinhasapredictedMWof99.97kDa.Duetoglycosylation,theproteinmigratesto110-130kDabasedonSDS-PAGEresult.(Endotoxin)Lessthan1EUperμgbytheLALmethod.純度(Purity)>95%asdeterminedbySDS-PAGEandHPLC.制劑(Formulation)Lyophilizedfrom0.22μmfilteredsolutioninPBS(pH7.4).Normally8%trehaloseisaddedasprotectantbeforelyophilization.重構方法(Reconstitution)Centrifugethetubebeforeopening.Reconstitutingtoaconcentrationmorethan100μg/mlisrecommended.Dissolvethelyophilizedproteinindistilledwater.儲存條件Theproductshouldbestoredat-25~-15℃for1yearfromdateofreceipt.2-7days,2~8°Cundersterileconditionsafterreconstitution.3-6months,-85~-65℃understerileconditionsafterreconstitution.然而,,在肝臟中,,IL-28A誘導的Th1細胞因子反應有助于T細胞介導的肝炎的炎癥。
Trop-2,alsoknownasepithelialglycoprotein-1antigen(EGP-1),isaproteinthatinhumansisencodedbytheTACSTD2gene.Mutationsofthisgeneresultingelatinousdrop-likecornealdystrophy,anautosomalrecessivedisordercharacterizedbyseverecornealamyloidosisleadingtoblindness.產品性質別名EGP1;EGP-1;TROP2;GA733-1;gp50;T16;TACSTD2;TROP-2;M1S1;TACD2UniprotNo.P09758表達區(qū)間及表達系統(tǒng)RecombinantBiotinylatedHumanTROP-2/TACSTD2ProteinisexpressedfromHEK293cellswithHistagandAvitagattheC-terminal.ItcontainsHis27-Thr274.分子量TheproteinhasapredictedMWof30.5kDa.Duetoglycosylation,theproteinmigratesto46-55kDabasedonTris-BisPAGEresult.純度>95%asdeterminedbySDS-PAGEandHPLC活性ELISAData:ImmobilizedAnti-TROP-2Antibody,hFcTagat0.5μg/mL(100μL/well)ontheplate.DoseresponsecurveforBiotinylatedHumanTROP-2,HisTagwiththeEC50of24.1ng/mLdeterminedbyELISA.0EUper1μgoftheproteinbytheLALmethod.制劑Lyophilizedfrom0.22μmfilteredsolutioninPBS(pH7.4).Normally5%trehaloseisaddedasprotectantbeforelyophilization.Kex2不能識別和切割單一堿性氨基酸即精氨酸或賴氨酸的羧基端肽鍵,。PNGase F (N-糖酰胺酶F或肽N-糖苷酶 F)
OSM與白血病抑制因子(LIF)密切相關,,實際上,,除了其在人類中的特定受體外,它實際上還使用了LIF受體,。Recombinant Mouse Fc gamma RIIB/CD32b Protein,His Tag
提供來源于牛肺的抑肽酶(動物源性),。產品信息規(guī)格1mg/10mg產品性質來源(Source)大腸桿菌表達CAS號(CASNO.)9087-70-1外觀(Appearance)白色、類白色,、類黃色粉末溶解度(5mg/mlin0.9%NaCl)可溶,,澄清或微濁純度(Purity)≥95%(HPLC)蛋白含量(Proteincontent)≥60%酶濃度(EnzymeConcentration)≥3.0EPU/mgpro活性定義(ActivityDefinition)胰蛋白酶單位:每秒鐘能水解1μmoL的N-苯甲酰-L-精氨酸乙酯(BAEE)為1個胰蛋白酶單位。酶活單位:能抑制1個胰蛋白酶單位的活力稱為1個抑肽酶活力單位(EPU),。儲存條件凍干粉2~8℃保存,,有效期2年。使用方法抑肽酶與蛋白酶是等摩爾有效結合,,推薦的結合pH>6.0,,在pH<3.0的條件下不結合??芍苯邮褂?.9%NaCl溶解,溶解后可-20℃儲存,。Recombinant Mouse Fc gamma RIIB/CD32b Protein,His Tag