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Recombinant Human IL-17Rc Protein

來源: 發(fā)布時間:2024-04-23

Recombinant Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVGAVGVGK) Monomer Protein,His-Avi Tag性能參數(shù)分子別名(Synonyms)MHC;KRAS;K-Ras2;KRAS2;C-K-RAS;CFC2;K-RAS2A;K-RAS2B;K-RAS4A;K-RAS4B;KRAS1;KRAS2;NS;NS3;RASK2;GTPaseKras;KI-RAS;RALD表達區(qū)間及表達系統(tǒng)(Source)BiotinylatedHumanHLA-A*03:01&B2M&KRASG12V(VVGAVGVGK)MonomerProteinisexpressedfromHEK293withHistagandAvitagattheC-TerminusItcontainsGly25-Thr305(HLA-A*03:01),Ile21-Met119(B2M)andVVGAVGVGKpeptide.[Accession|NP_002107.3(HLA-A*03:01)&P61769(B2M)&VVGAVGVGK]分子量大?。∕olecularWeight)TheproteinhasapredictedMWof50.09kDa.Duetoglycosylation,theproteinmigratesto51-60kDabasedonSDS-PAGEresult.(Endotoxin)Lessthan1EUperμgbytheLALmethod.純度(Purity)>95%asdeterminedbySDS-PAGEandHPLC.制劑(Formulation)Suppliedas0.22μmfilteredsolutioninPBS(pH7.4).SDF-1同工型與細胞表面上的CXCR4和CXCR7受體相互作用,也可以結合Syndecan-4,。Recombinant Human IL-17Rc Protein,hFc Tag

Recombinant Human IL-17Rc Protein,hFc Tag,標準物質(zhì)

Name:AITRL,MouseSynonyms:Activation-inducedTNFRmemberLigand,TNFSF18,GITRL,TL-6Description:Activation-InducibleTNF-RelatedLigand(AITRL),alsoknownasGlucocorticoid-InducedTNF-RelatedLigand(GITRL),belongstothetumornecrosisfactorsuperfamily(TNFSF).AITRLisaTypeIIsingletransmembraneproteinandshareslowconservationwithintheextracellulardomainwithotherTNFSFmembers.AITRLisexpressedonmacrophages,immatureandmaturedendriticcellsandBcells.Itsreceptor,Activation-InducibleTNFRfamilyReceptor(AITR),isexpressedonTlymphocytes,naturalkiller(NK)cells,andantigen-presentingcells.AfterbindingbyAITRL,AITRcanbereleased.AITRactivationincreasesresistancetotumorsandviralinfectionsandisinvolvedinautoimmuneandinflammatoryprocesses.Inaddition,activatedAITRincreasesTCR-inducedTcellproliferationandcytokineproductionandrescuesTcellsandNKcellsfromapoptosis.RecombinantmouseActivation-InducibleTNF-RelatedLigand(rmAITRL)producedinE.Recombinant Mouse EMAP-II/AIMP1 Protein,His TagCX3CL1還通過募集巨噬細胞以及通過募集和介導破骨細胞前體的粘附而導致傷口愈合,。

Recombinant Human IL-17Rc Protein,hFc Tag,標準物質(zhì)

Trop-2,alsoknownasepithelialglycoprotein-1antigen(EGP-1),isaproteinthatinhumansisencodedbytheTACSTD2gene.Mutationsofthisgeneresultingelatinousdrop-likecornealdystrophy,anautosomalrecessivedisordercharacterizedbyseverecornealamyloidosisleadingtoblindness.產(chǎn)品性質(zhì)別名EGP1;EGP-1;TROP2;GA733-1;gp50;T16;TACSTD2;TROP-2;M1S1;TACD2UniprotNo.P09758表達區(qū)間及表達系統(tǒng)RecombinantBiotinylatedHumanTROP-2/TACSTD2ProteinisexpressedfromHEK293cellswithHistagandAvitagattheC-terminal.ItcontainsHis27-Thr274.分子量TheproteinhasapredictedMWof30.5kDa.Duetoglycosylation,theproteinmigratesto46-55kDabasedonTris-BisPAGEresult.純度>95%asdeterminedbySDS-PAGEandHPLC活性ELISAData:ImmobilizedAnti-TROP-2Antibody,hFcTagat0.5μg/mL(100μL/well)ontheplate.DoseresponsecurveforBiotinylatedHumanTROP-2,HisTagwiththeEC50of24.1ng/mLdeterminedbyELISA.0EUper1μgoftheproteinbytheLALmethod.制劑Lyophilizedfrom0.22μmfilteredsolutioninPBS(pH7.4).Normally5%trehaloseisaddedasprotectantbeforelyophilization.

糖苷酶F(PNGaseF)是一種酰胺水解酶,經(jīng)過和平空間站伊麗莎菌克隆,,主要由腦膜炎膿桿菌等革蘭氏陰性菌分泌,。N-糖苷酶F(PNGaseF)在酵母中重組表達(比活性:100000U/mL),,可以裂解由天冬酰胺連接的高甘露糖、雜合和復雜的寡糖糖蛋白,。PNGaseF的切割位點為糖蛋白內(nèi)側N-乙酰葡萄糖胺(GlcNAc)和天冬酰氨殘基之間的酰胺鍵,,同時將酶解后蛋白上的天冬氨酰轉化為天冬氨酸。本產(chǎn)品帶his標簽,,常應用于抗體及其相關蛋白完全去糖基化,。另外,我司還提供其他類型的糖苷酶,,包括酵母重組表達的N-糖苷酶F(比活性:750000U/mL),,內(nèi)切糖苷酶H,內(nèi)切糖苷酶S,。產(chǎn)品信息產(chǎn)品性質(zhì)中文別名(Chinesesynonym)N-糖酰胺酶F,;N-糖苷酶F英文別名(Englishsynonym)PNGaseF來源(Source)酵母重組表達分子量(Molecularweight)36kDa比活性(Specificactivity)100000U/mL緩沖液組分(Buffer)20mMTris-HClpH7.5,50mMNaCl,5mMEDTA,50%Glycerol內(nèi)分泌腺衍生的血管內(nèi)皮生長因子(EG-VEGF),也稱為(PK1),,是分泌蛋白的原蛋白蛋白家族的成員,。

Recombinant Human IL-17Rc Protein,hFc Tag,標準物質(zhì)

N-糖苷酶 F (PNGase F)酶活定義(UnitDefinition)1個酶活力單位指在10μL的反應體系中,37℃條件下1小時從10μg變性RNaseB中除去超過95%的碳水化合物所需要的酶量,。儲存條件-15~-25℃保存,,有效期1年。使用說明變性條件下蛋白質(zhì)去糖基化1)在水中加入1μLBuffer1和目標糖蛋白(1-20μg),,至終體積10μL,;2)100℃溫度下煮沸10min使其變性,冰上冷卻,,離心10秒,;3)加入2μL的Buffer2、2μL的10%NP-40,、6μL去離子水,,總反應體積20μL;4)加入0.2~0.5μL的PNGase,,輕輕混勻,。在37℃孵育1-3h。非變性條件下蛋白質(zhì)去糖基化1)在水中加入2μL的Buffer2和目標糖蛋白(1-20μg)至體積為20μL,。2)加入0.5~1μL的PNGaseF,輕輕混勻,。3)37°C孵育4-24h。注意:在變性條件下大多數(shù)底物能夠更好的去糖基化,,在非變性條件下可能需要增加PNGaseF的量和延長孵育時間,。RANTES是對單核細胞,記憶T細胞(CD4+/CD45RO),嗜堿性粒細胞和嗜酸性粒細胞的趨化劑,。Recombinant Cynomolgus PD-L2/B7-DC Protein,His Tag

IdeS 具有獨特的底物選擇性,,可以作為工具酶應用于抗體類藥物或抗體融合蛋白藥物的結構表征分析。Recombinant Human IL-17Rc Protein,hFc Tag

性能參數(shù)表達區(qū)間及表達系統(tǒng)(Source)CynomolgusCD27Ligand/CD70ProteinisexpressedfromHEK293withHistagattheN-terminal.ItcontainsGln39-Pro194.[Accession|G7PYU6-1]分子量大?。∕olecularWeight)TheproteinhasapredictedMWof18.4kDa.Duetoglycosylation,theproteinmigratesto60-90kDabasedonTris-BisPAGEresult.(Endotoxin)Lessthan1EUperugbytheLALmethod.純度(Purity)>95%asdeterminedbyTris-BisPAGE活性(Activity)ELISAData:ImmobilizedCynomolgusCD27Ligand,HisTagat2μg/ml(100μl/well)ontheplate.DoseresponsecurveforCynomolgus/RhesusmacaqueCD27,hFcTagwiththeEC50of70.8ng/mldeterminedbyELISA.制劑(Formulation)Lyophilizedfrom0.22μmfilteredsolutioninPBS(pH7.4).Normally8%trehaloseisaddedasprotectantbeforelyophilization.重構方法(Reconstitution)Centrifugetubesbeforeopening.Reconstitutingtoaconcentrationmorethan100μg/mlisrecommended.Dissolvethelyophilizedproteinindistilledwater.Recombinant Human IL-17Rc Protein,hFc Tag